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BRIP1/FANCJ Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14361

Novus Biologicals, part of Bio-Techne
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NBP2-14361
NBP2-14361-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for BRIP1/FANCJ Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: FNKQTKRVSWSSFNSLGQYFTGKIPKATPELGSSENSASSPPRFKTEKMESKTVLPFTDKCESSNLTVNTSFGSCPQSETIISSLKIDATLTRKNHSEHPLCSEEALDPDIELSLVSEEDKQSTSNRDFETEAEDESIYFTPEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRIP1/FANCJ Antibody

Western Blot: BRIP1/FANCJ Antibody [NBP2-14361]

Western Blot: BRIP1/FANCJ Antibody [NBP2-14361]

Western Blot: BRIP1/FANCJ Antibody [NBP2-14361] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRIP1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP2-14361]

Immunocytochemistry/ Immunofluorescence: BRIP1/FANCJ Antibody [NBP2-14361]

Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP2-14361] - Staining of human cell line A-431 shows positivity in nucleus and nuclear membrane.
Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP2-14361]

Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP2-14361]

Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP2-14361] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Applications for BRIP1/FANCJ Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRIP1/FANCJ

The Fanconi anemia group subunit J protein (FANCJ), also called BRIP1 or BACH1, is a helicase that interacts with the BRCT domain of BRCA1 and has a role in BRCA1-dependent DNA repair and checkpoint. Defects in FANCJ cause the autosomal recessive disorder, Fanconi anemia. BRIP1/FANCJ has a function in the Fanconi anemia pathway that is independent of BRCA1 and downstream of FANCD2 activation.

FANCG antibodies are useful for DNA repair studies and cell cycle research.

Long Name

BRCA1 Interacting Protein C-terminal Helicase 1

Alternate Names

BACH1, FACJ, FANCJ

Gene Symbol

BRIP1

Additional BRIP1/FANCJ Products

Product Documents for BRIP1/FANCJ Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRIP1/FANCJ Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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