BRIX Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99257
![Western Blot: BRIX Antibody [NBP2-99257]](https://resources.bio-techne.com/images/products/BRIX-Antibody-Western-Blot-NBP2-99257-img0002.jpg "Western Blot: BRIX Antibody [NBP2-99257]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for BRIX Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human BRIX.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for BRIX Antibody
Western Blot: BRIX Antibody [NBP2-99257]
Western Blot: BRIX Antibody [NBP2-99257] - Anti-BRIX rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 43 kDa![Immunocytochemistry/ Immunofluorescence: BRIX Antibody [NBP2-99257]](https://resources.bio-techne.com/images/products/BRIX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99257-img0001.jpg "Immunocytochemistry/ Immunofluorescence: BRIX Antibody [NBP2-99257]")
Immunocytochemistry/ Immunofluorescence: BRIX Antibody [NBP2-99257]
Immunocytochemistry/Immunofluorescence: BRIX Antibody [NBP2-99257] - Immunofluorescence staining of BRIX in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BRIX polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Nucleolus .Applications for BRIX Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:300-1:10000
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BRIX
Long Name
Brix Domain Containing 2
Alternate Names
BRIX1, BXDC2
Gene Symbol
BRIX1
Additional BRIX Products
Product Specific Notices for BRIX Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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