Brk Antibody (2H12B8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60537

Novus Biologicals, part of Bio-Techne
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NB110-60537
NB110-60537-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2H12B8

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Brk Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Brk expressed in E. Coli.

Specificity

PTK6 (2H12B8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Brk Antibody (2H12B8) - BSA Free

Western Blot: Brk Antibody (2H12B8)BSA Free [NB110-60537]

Western Blot: Brk Antibody (2H12B8)BSA Free [NB110-60537]

Western Blot: Brk Antibody (2H12B8) [NB110-60537] - Analysis using PTK6 mouse mAb against Hela (1), A549 (2) and MCF-7 (3) cell lysate.
Brk Antibody (2H12B8) - BSA Free

Western Blot: Mouse Monoclonal Brk Antibody (2H12B8) [NB110-60537]

Western Blot: Mouse Monoclonal Brk Antibody (2H12B8) [NB110-60537] - Whole cell lysates from MDA-MB-231 cells that were exposed to 20% or 1% O2 for 48 hours were loaded with 50 ug/lane. 10% SDS-PAGE. Brk Antibody (NB110-60537) was used for primary antibody: 1:1000, 4℃, overnight. Image from a verified customer review.

Applications for Brk Antibody (2H12B8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 1 review rated 5 using NB110-60537 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Brk

PTK6 (protein tyrosine kinase 6, BRK or FLJ42088),with 451-amino acid protein (about 51kDa), encods a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphory-lation. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. And Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes, but not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Over expression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype.

Long Name

Breast Tumor Kinase

Alternate Names

PTK6, Sik, Tksk

Entrez Gene IDs

5753 (Human)

Gene Symbol

PTK6

Additional Brk Products

Product Documents for Brk Antibody (2H12B8) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Brk Antibody (2H12B8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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