Brk [p Tyr342] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29687

Novus Biologicals, part of Bio-Techne
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NBP2-29687
NBP2-29687-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Brk Antibodies »

Product Summary for Brk [p Tyr342] Antibody

Immunogen

KLH conjugated synthetic peptide corresponding to amino acids encompassing Tyr342 of human Brk.

Modification

p Tyr342

Specificity

Recognizes human phospho-Brk at tyrosine 342.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Relevant Entrez Gene Numbers: NM_005975.2

Scientific Data Images for Brk [p Tyr342] Antibody

Western Blot: Brk [p Tyr342] Antibody [NBP2-29687]

Western Blot: Brk [p Tyr342] Antibody [NBP2-29687]

Western Blot: Brk [p Tyr342] Antibody [NBP2-29687] - IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk(y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(y342).

Applications for Brk [p Tyr342] Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M Tris-Glycine (pH 7.4) and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Brk

Brk is encoded by this gene is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. The encoded protein has been shown to undergo autophosphorylation. [provided by RefSeq]

Long Name

Breast Tumor Kinase

Alternate Names

PTK6, Sik, Tksk

Gene Symbol

PTK6

UniProt

Q13882

Additional Brk Products

Product Documents for Brk [p Tyr342] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Brk [p Tyr342] Antibody

Relevant Entrez Gene Numbers: NM_005975.2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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