BRM Antibody (2D12)

Novus Biologicals, part of Bio-Techne | Catalog # H00006595-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BRM Antibodies »

Product Specifications

Immunogen

SMARCA2 (NP_003061, 659 a.a. ~ 735 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KILLDPNSEEVSEKDAKQIIETAKQDVDDEYSMQYSARGSQSYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWM

Specificity

SMARCA2 - SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for BRM Antibody (2D12)

Immunocytochemistry/ Immunofluorescence: BRM Antibody (2D12) [H00006595-M06]

Immunocytochemistry/ Immunofluorescence: BRM Antibody (2D12) [H00006595-M06]

Immunocytochemistry/Immunofluorescence: BRM Antibody (2D12) [H00006595-M06] - Analysis of monoclonal antibody to SMARCA2 on HeLa cell. Antibody concentration 10 ug/ml
ELISA: BRM Antibody (2D12) [H00006595-M06]

ELISA: BRM Antibody (2D12) [H00006595-M06]

ELISA: BRM Antibody (2D12) [H00006595-M06] - Detection limit for recombinant GST tagged SMARCA2 is approximately 0.1ng/ml as a capture antibody.

Applications for BRM Antibody (2D12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BRM

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

Long Name

Probable global transcription activator SNF2L2

Alternate Names

BAF190B, EC 3.6.4.-, hBRM, SMARCA2, SNF2-alpha, SNF2A, SNF2L2

Entrez Gene IDs

6595 (Human)

Gene Symbol

SMARCA2

OMIM

600014 (Human)

UniProt

NP_003061 (Human)

Additional BRM Products

Product Documents for BRM Antibody (2D12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRM Antibody (2D12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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