BRM Antibody (HL1115)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13725
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1115
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human BRM. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for BRM Antibody (HL1115)
![Immunocytochemistry/ Immunofluorescence: BRM Antibody (HL1115) [NBP3-13725]](https://resources.bio-techne.com/images/products/BRM-Antibody-HL1115-Immunocytochemistry-Immunofluorescence-NBP3-13725-img0004.jpg "Immunocytochemistry/ Immunofluorescence: BRM Antibody (HL1115) [NBP3-13725]")
Immunocytochemistry/ Immunofluorescence: BRM Antibody (HL1115) [NBP3-13725]
Immunocytochemistry/Immunofluorescence: BRM Antibody (HL1115) [NBP3-13725] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCA2 stained by SMARCA2 antibody [HL1115] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.![Immunohistochemistry-Paraffin: BRM Antibody (HL1115) [NBP3-13725]](https://resources.bio-techne.com/images/products/BRM-Antibody-HL1115-Immunohistochemistry-Paraffin-NBP3-13725-img0002.jpg "Immunohistochemistry-Paraffin: BRM Antibody (HL1115) [NBP3-13725]")
Immunohistochemistry-Paraffin: BRM Antibody (HL1115) [NBP3-13725]
Immunohistochemistry-Paraffin: BRM Antibody (HL1115) [NBP3-13725] - Mouse brain. BRM stained by BRM antibody [HL1115] (NBP3-13725) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
BRM Antibody (HL1115) [NBP3-13725] - Rat brain. SMARCA2 stained by SMARCA2 antibody [HL1115] diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Applications for BRM Antibody (HL1115)
Application
Recommended Usage
Western Blot
1:500-1:3000
Application Notes
ICC/IF, IHC-P- Assay depenendent
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BRM
Long Name
Probable global transcription activator SNF2L2
Alternate Names
BAF190B, EC 3.6.4.-, hBRM, SMARCA2, SNF2-alpha, SNF2A, SNF2L2
Gene Symbol
SMARCA2
Additional BRM Products
Product Documents for BRM Antibody (HL1115)
Product Specific Notices for BRM Antibody (HL1115)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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