BRM Antibody (PSH01-15)

Western Blot: BRM Antibody (PSH01-15) [NBP3-32957]

Western Blot: BRM Antibody (PSH01-15) [NBP3-32957] - Western blot analysis of BRM on different lysates with Rabbit anti-BRM antibody (NBP3-32957) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NCCIT cell lysate (low expression)

Lysates/proteins at 30 ug/Lane.

Predicted band size: 181 kDa
Observed band size: 200 kDa

Exposure time: 3 minutes 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32957) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: BRM Antibody (PSH01-15) [NBP3-32957]

Immunohistochemistry: BRM Antibody (PSH01-15) [NBP3-32957] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-BRM antibody (NBP3-32957) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32957) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: BRM Antibody (PSH01-15) [NBP3-32957]

Flow Cytometry: BRM Antibody (PSH01-15) [NBP3-32957] - Flow cytometric analysis of MCF7 cells labeling BRM.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32957, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).