BRM Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-55309
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Immunoprecipitation, QcWestern, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1325 and 1375 of human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 using the numbering given in entry CAA51407.1 (GeneID 6595)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BRM Antibody
![Western Blot: BRM Antibody [NB100-55309]](https://resources.bio-techne.com/images/products/BRM-Antibody-NB100-55309-img0003.jpg "Western Blot: BRM Antibody [NB100-55309]")
Western Blot: BRM Antibody [NB100-55309]
Western Blot: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).![Immunoprecipitation: BRM Antibody [NB100-55309]](https://resources.bio-techne.com/images/products/BRM-Antibody-Immunoprecipitation-NB100-55309-img0004.jpg "Immunoprecipitation: BRM Antibody [NB100-55309]")
Immunoprecipitation: BRM Antibody [NB100-55309]
Immunoprecipitation: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. SMARCA2/BRM was also immunoprecipitated by rabbit anti-SMARCA2/BRM antibodies NB100-55307 and NB100-55308, which recognize upstream epitopes. For blotting immunoprecipitated SMARCA2/BRM, NB100-55309 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.![QcWestern: BRM Antibody [NB100-55309]](https://resources.bio-techne.com/images/products/BRM-Antibody-QcWestern-NB100-55309-img0005.jpg "QcWestern: BRM Antibody [NB100-55309]")
QcWestern: BRM Antibody [NB100-55309]
QcWestern: BRM Antibody [NB100-55309] - Detection of human SMARCA2/BRM by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-SMARCA2/BRM antibody NB100-55309 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.Applications for BRM Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 5 ug/mg lysate
Western Blot
1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BRM
Long Name
Probable global transcription activator SNF2L2
Alternate Names
BAF190B, EC 3.6.4.-, hBRM, SMARCA2, SNF2-alpha, SNF2A, SNF2L2
Entrez Gene IDs
6595 (Human)
Gene Symbol
SMARCA2
UniProt
Additional BRM Products
Product Documents for BRM Antibody
Product Specific Notices for BRM Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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