BRN3B/POU4F2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26061
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Summary for BRN3B/POU4F2 Antibody
Immunogen
Peptide with sequence HGLPSHMGCMSDVD corresponding to internal region according to NP_004566.2.
Specificity
This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.
Scientific Data Images for BRN3B/POU4F2 Antibody
Western Blot: BRN3B/POU4F2 Antibody [NBP2-26061] -
Western Blot: BRN3B/POU4F2 Antibody [NBP2-26061] - (1ug/ml) staining of Human Cerebellum (A) + peptide (B), Mouse Brain (C) + peptide (D), and Rat Brain (E) + peptide (F) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for BRN3B/POU4F2 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
1-3 ug/ml
Application Notes
Approx. 35kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 43.1kDa according to Human NP_004566.2). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BRN3B/POU4F2
Alternate Names
BRN3.2, BRN3B, Brn-3b, POU class 4 homeobox 2, POU domain, class 4, transcription factor 2
Entrez Gene IDs
5458 (Human)
Gene Symbol
POU4F2
Additional BRN3B/POU4F2 Products
Product Documents for BRN3B/POU4F2 Antibody
Product Specific Notices for BRN3B/POU4F2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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