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BRN3B/POU4F2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26061

Novus Biologicals, part of Bio-Techne
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NBP2-26061

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for BRN3B/POU4F2 Antibody

Immunogen

Peptide with sequence HGLPSHMGCMSDVD corresponding to internal region according to NP_004566.2.

Specificity

This antibody is not expected to cross-react to the similar POU4F3 (GeneID 5459)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Please note, this antibody is considered to be "Innovators Grade". Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.

Scientific Data Images for BRN3B/POU4F2 Antibody

BRN3B/POU4F2 Antibody

Western Blot: BRN3B/POU4F2 Antibody [NBP2-26061] -

Western Blot: BRN3B/POU4F2 Antibody [NBP2-26061] - (1ug/ml) staining of Human Cerebellum (A) + peptide (B), Mouse Brain (C) + peptide (D), and Rat Brain (E) + peptide (F) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for BRN3B/POU4F2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1-3 ug/ml
Application Notes
Approx. 35kDa band observed in Human, Mouse and Rat Brain lysates (calculated MW of 43.1kDa according to Human NP_004566.2). This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BRN3B/POU4F2

POU4F2 is a member of the POU-domain family of transcription factors. POU-domain proteins have been observed to play important roles in control of cell identity in several systems. A class IV POU-domain protein, POU4F2 is found in human retina exclusively within a subpopulation of ganglion cells where it may play a role in determining or maintaining the identities of a small subset of visual system neurons.(supplied by OMIM)

Alternate Names

BRN3.2, BRN3B, Brn-3b, POU class 4 homeobox 2, POU domain, class 4, transcription factor 2

Entrez Gene IDs

5458 (Human)

Gene Symbol

POU4F2

Additional BRN3B/POU4F2 Products

Product Documents for BRN3B/POU4F2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRN3B/POU4F2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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