Bromodeoxyuridine/BrdU Antibody (BRD.3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45221
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) [NBP2-45221]](https://resources.bio-techne.com/images/products/Bromodeoxyuridine-BrdU-Antibody-BRD-3-Immunohistochemistry-Paraffin-NBP2-45221-img0001.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) [NBP2-45221]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Non-species specific
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BRD.3
Concentration
0.2 mg/ml
Product Summary for Bromodeoxyuridine/BrdU Antibody (BRD.3)
Immunogen
Bromodeoxyuridine/BrdU conjugated to BSA
Localization
Nuclear
Specificity
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Marker
Proliferation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-92989)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Bromodeoxyuridine/BrdU Antibody (BRD.3)
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) [NBP2-45221]
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD.3) [NBP2-45221] - Mouse Small Intestine stained with BrdU Monoclonal Antibody (BRD.3).Applications for Bromodeoxyuridine/BrdU Antibody (BRD.3)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Bromodeoxyuridine/BrdU
Alternate Names
5-bromo-2-deoxyuridine, BrdU
Additional Bromodeoxyuridine/BrdU Products
Product Documents for Bromodeoxyuridine/BrdU Antibody (BRD.3)
Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (BRD.3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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