Key Product Details

Species Reactivity

Validated:

Non-species specific

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BRD494

Concentration

0.2 mg/ml

View all Bromodeoxyuridine/BrdU Antibodies »

Product Specifications

Immunogen

Bromodeoxyuridine/BrdU conjugated to KLH

Localization

Nuclear

Specificity

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Marker

Proliferation Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34566)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Bromodeoxyuridine/BrdU Antibody (BRD494)

Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD494) [NBP2-32920]

Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD494) [NBP2-32920]

Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BRD494) [NBP2-32920] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Monoclonal Antibody (BRD494).

Applications for Bromodeoxyuridine/BrdU Antibody (BRD494)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1 ug/ml

Immunohistochemistry

0.5-1 ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml
Application Notes
Immunohistology (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Bromodeoxyuridine/BrdU

Bromodeoxyuridine (BrdU) is a synthetic nucleoside analogue of thymidine which can be incorporated into DNA in proliferating cells. During mitosis, BrdU may be incorporated into the newly synthezised DNA of S-phase cells, and can therefore provide an estimate for the fraction of cells in S-phase. Labeling cells with BrdU and then screening with BrdU antibodies has become a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo, BrdU is considered a potential carcinogen, as its incorporation into DNA may cause mutations.

Alternate Names

5-bromo-2-deoxyuridine, BrdU

Additional Bromodeoxyuridine/BrdU Products

Product Documents for Bromodeoxyuridine/BrdU Antibody (BRD494)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (BRD494)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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