Bromodeoxyuridine/BrdU Antibody (BU20a) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34567
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BU20a) - Azide and BSA Free [NBP2-34567]](https://resources.bio-techne.com/images/products/Bromodeoxyuridine-BrdU-Antibody-BU20a-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34567-img0001.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BU20a) - Azide and BSA Free [NBP2-34567]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Non-species specific
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # BU20a
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Bromodeoxyuridine/BrdU Antibody (BU20a) - Azide and BSA Free
Immunogen
Bromodeoxyuridine/BrdU conjugated to KLH
Localization
Nuclear
Specificity
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Marker
Proliferation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32922).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Bromodeoxyuridine/BrdU Antibody (BU20a) - Azide and BSA Free
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BU20a) - Azide and BSA Free [NBP2-34567]
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (BU20a) - Azide and BSA Free [NBP2-34567] - Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Monoclonal Antibody (BU20a).Applications for Bromodeoxyuridine/BrdU Antibody (BU20a) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Bromodeoxyuridine/BrdU
Alternate Names
5-bromo-2-deoxyuridine, BrdU
Additional Bromodeoxyuridine/BrdU Products
Product Documents for Bromodeoxyuridine/BrdU Antibody (BU20a) - Azide and BSA Free
Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (BU20a) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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