Bromodeoxyuridine/BrdU Antibody (MoBu-1)
Novus Biologicals, part of Bio-Techne | Catalog # NB500-439
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439]](https://resources.bio-techne.com/images/products/Bromodeoxyuridine-BrdU-Antibody-MoBu-1-Immunohistochemistry-Paraffin-NB500-439-img0003.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Non-species specific
Cited:
Human, Mouse, Non-species specific
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MoBu-1
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Bromodeoxyuridine/BrdU conjugated to hemocyanine (isolated from Helix pomatia)
Localization
Nuclear
Specificity
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Marker
Proliferation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-68041)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Bromodeoxyuridine/BrdU Antibody (MoBu-1)
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439]
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439] - Mouse Small Intestine stained with BrdU Monoclonal Antibody (MoBu-1).![Immunohistochemistry: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439]](https://resources.bio-techne.com/images/products/Bromodeoxyuridine-BrdU-Antibody-MoBu-1-Immunohistochemistry-NB500-439-img0002.jpg "Immunohistochemistry: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439]")
Immunohistochemistry: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439]
Immunohistochemistry: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439] - Staining of bromodeoxyuridine/BrdU-labeled cells (chick embryo; paraffin-embedded sections)Applications for Bromodeoxyuridine/BrdU Antibody (MoBu-1)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Bromodeoxyuridine/BrdU
Alternate Names
5-bromo-2-deoxyuridine, BrdU
Additional Bromodeoxyuridine/BrdU Products
Product Documents for Bromodeoxyuridine/BrdU Antibody (MoBu-1)
Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (MoBu-1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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