Bromodeoxyuridine/BrdU Antibody (SPM166) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34744
![Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (SPM166) - Azide and BSA Free [NBP2-34744]](https://resources.bio-techne.com/images/products/Bromodeoxyuridine-BrdU-Antibody-SPM166-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34744-img0001.jpg "Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (SPM166) - Azide and BSA Free [NBP2-34744]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Non-species specific
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM166
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Bromodeoxyuridine/BrdU Antibody (SPM166) - Azide and BSA Free
Immunogen
Bromodeoxyuridine/BrdU conjugated to KLH
Localization
Nuclear
Specificity
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Marker
Proliferation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
307 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32919).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Bromodeoxyuridine/BrdU Antibody (SPM166) - Azide and BSA Free
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (SPM166) - Azide and BSA Free [NBP2-34744]
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (SPM166) - Azide and BSA Free [NBP2-34744] - Formalin-fixed, paraffin-embedded mouse intestine tissue stained with BrdU MAb (SPM166).Applications for Bromodeoxyuridine/BrdU Antibody (SPM166) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Use Ab at 1ug/ml for 30 minute at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Bromodeoxyuridine/BrdU
Alternate Names
5-bromo-2-deoxyuridine, BrdU
Additional Bromodeoxyuridine/BrdU Products
Product Documents for Bromodeoxyuridine/BrdU Antibody (SPM166) - Azide and BSA Free
Product Specific Notices for Bromodeoxyuridine/BrdU Antibody (SPM166) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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