Bromodeoxyuridine/BrdU Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB500-235

Novus Biologicals, part of Bio-Techne
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NB500-235
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, All Species, Guinea Pig

Cited:

Human, Mouse, Rat, Guinea Pig, Rabbit

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, In vitro assay

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Concentration

3.2 mg/ml

View all Bromodeoxyuridine/BrdU Antibodies »

Product Specifications

Immunogen

Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH).

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33731750).

Specificity

BrdU

Marker

Proliferation Marker

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Bromodeoxyuridine/BrdU Antibody

Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235]

Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235]

Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235] - IF analysis of BrdU in Proteinase K pretreated mouse lacrimal gland. Image courtesy of anonymous customer review.
Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235]

Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235]

Immunoprecipitation: Bromodeoxyuridine/BrdU Antibody [NB500-235] - Lysate was immunoprecipitated with anti-brdu antibody (NB500-235), 5-methyl cytosine (5-Me), or a no antibody control (No A). An ELISA was then performed to measure total activity.

Applications for Bromodeoxyuridine/BrdU Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

25-100 ug/ml
Application Notes
Use in IHC-Fr reported in scientific literature (PMID:33731750). Use in IHC-FrFl reported in scientific literature (PMID:33744708) In vitro reported in (PMID: 29100301).

Reviewed Applications

Read 2 reviews rated 5 using NB500-235 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

3.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Bromodeoxyuridine/BrdU

BrdU (Bromodeoxyuridine) is a synthetic nucleoside that is incorporated into actively replicating cells' DNA. In many studies, BrdU incorporation is used as a marker for the proliferation of cells. This technique is particularly valuable in detecting proliferation in tissues that are not easily accessible to other assay types, like the brain. It is also commonly used to detect developmental progress in embryogenesis. Detection of the concentration and pattern of BrdU incorporation in tissues can give clues as to the pattern, rate, and progression of cell proliferation.

Alternate Names

5-bromo-2-deoxyuridine, BrdU

Additional Bromodeoxyuridine/BrdU Products

Product Documents for Bromodeoxyuridine/BrdU Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Bromodeoxyuridine/BrdU Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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