Human peripheral blood mononuclear cells (PBMCs) were treated overnight with 50 ng/mL PMA, 500 ng/mL Ionomycin, and 30 µg/mL BrdU, then stained with Mouse Anti-Bromodeoxyuridine/BrdU APC-conjugated Monoclonal Antibody (Catalog # IC7225A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with cold, 70% ethanol for 5 minutes, DNA was denatured with 1.5M HCl for 30 minutes, and then cells were permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).View our protocol for Staining Intracellular Molecules.

Applications for Bromodeoxyuridine/BrdU APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs), treated with PMA, Ionomycin, and BrdU, were fixed with ethanol, DNA was denatured with HCl, and then cells were permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Bromodeoxyuridine/BrdU

Bromodeoxyuridine (BrdU) is a nucleoside analog that is incorporated into DNA in place of thymidine. BrdU differs from thymidine by virtue of the fact that BrdU shows a bromine atom substitution for a methyl group in thymidine. The detection of newly synthesized DNA containing BrdU is a commonly used measure of cell proliferation and progression through S phase of the cell cycle.

Alternate Names

5-bromo-2-deoxyuridine, BrdU

Additional Bromodeoxyuridine/BrdU Products

Product Documents for Bromodeoxyuridine/BrdU APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Bromodeoxyuridine/BrdU APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Bromodeoxyuridine/BrdU APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC7225A

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all Bromodeoxyuridine/BrdU Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Bromodeoxyuridine/BrdU APC-conjugated Antibody

Detection of Bromodeoxyuridine/BrdU in Human PBMCs by Flow Cytometry.