Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all BRSK1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 28 amino acid synthetic peptide from near the carboxy terminus of human BRSK1. The immunogen is located within amino acids 720 - 770 of BRSK1. Amino Acid Squence: DEKNGAQTRPAGAPPRSLQPPPGRPDPE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRSK1 Antibody - BSA Free

Western Blot: BRSK1 AntibodyBSA Free [NBP1-76778]

Western Blot: BRSK1 AntibodyBSA Free [NBP1-76778]

Western Blot: BRSK1 Antibody [NBP1-76778] - Human brain tissue lysate with BRSK1 antibody at (A) 0.5 and (B) 1 ug/ml.
BRSK1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BRSK1 Antibody - BSA Free [NBP1-76778] -

Immunocytochemistry/ Immunofluorescence: BRSK1 Antibody - BSA Free [NBP1-76778] - Immunofluorescence of BRSK1 in Human Brain cells with BRSK1 antibody at 20 ug/mL.
BRSK1 Antibody - BSA Free

Immunohistochemistry: BRSK1 Antibody - BSA Free [NBP1-76778] -

Immunohistochemistry: BRSK1 Antibody - BSA Free [NBP1-76778] - Immunohistochemistry of BRSK1 in human brain tissue with BRSK1 antibody at 2.5 ug/mL.

Applications for BRSK1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRSK1

BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.

Alternate Names

BR serine/threonine kinase 1, EC 2.7.11, EC 2.7.11.1, FLJ43009, KIAA1811BR serine/threonine-protein kinase 1, protein kinase SAD1A, SAD1, SAD1 kinase, Serine/threonine-protein kinase SAD-B

Gene Symbol

BRSK1

UniProt

Q8TDC3 (Human)

Additional BRSK1 Products

Product Documents for BRSK1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRSK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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