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BRUNOL4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15622

Novus Biologicals, part of Bio-Techne
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NBP2-15622

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (99%), Mouse (98%), Rat (98%), Xenopus (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for BRUNOL4 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human BRUNOL4. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (97%).

Localization

Nucleus; Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BRUNOL4 Antibody

Western Blot: BRUNOL4 Antibody [NBP2-15622]

Western Blot: BRUNOL4 Antibody [NBP2-15622]

Western Blot: BRUNOL4 Antibody [NBP2-15622] - Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 7. 5% SDS PAGE gel, diluted at 1:3000.
Immunohistochemistry-Paraffin: BRUNOL4 Antibody [NBP2-15622]

Immunohistochemistry-Paraffin: BRUNOL4 Antibody [NBP2-15622]

Immunohistochemistry-Paraffin: BRUNOL4 Antibody [NBP2-15622] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

Applications for BRUNOL4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BRUNOL4

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Bruno (Drosophila) -like 4, RNA binding protein, BRUNOL-4, BRUNOL4Bruno -like 4, RNA binding protein, bruno-like 4, RNA binding protein, bruno-like 4, RNA binding protein (Drosophila), Bruno-like protein 4, CELF-4, CUG-BP and ETR-3 like factor 4, CUG-BP- and ETR-3-like factor 4, CUGBP Elav-like family member 4, CUGBP, Elav-like family member 4, LYST-interacting protein LIP9, RNA-binding protein BRUNOL4, RNA-binding protein BRUNOL-4

Gene Symbol

CELF4

UniProt

Additional BRUNOL4 Products

Product Documents for BRUNOL4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRUNOL4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.