BST2 Antibody (2E2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29621
![Immunohistochemistry-Paraffin: BST2 Antibody (2E2) - BSA Free [NBP2-29621]](https://resources.bio-techne.com/images/products/BST2-Antibody-2E2-Immunohistochemistry-Paraffin-NBP2-29621-img0003.jpg "Immunohistochemistry-Paraffin: BST2 Antibody (2E2) - BSA Free [NBP2-29621]")
![Western Blot: BST2 Antibody (2E2)BSA Free [NBP2-29621]](https://resources.bio-techne.com/images/products/BST2-Antibody-2E2-Western-Blot-NBP2-29621-img0001.jpg "Western Blot: BST2 Antibody (2E2)BSA Free [NBP2-29621]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E2
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for BST2 Antibody (2E2) - BSA Free
Immunogen
Full length recombinant human BST2 protein [NCBI NP_004326]
Localization
Cell membrane, trans-Golgi network, late endosome and cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for BST2 Antibody (2E2) - BSA Free
Western Blot: BST2 Antibody (2E2)BSA Free [NBP2-29621]
Western Blot: BST2 Antibody (2E2) [NBP2-29621] - Analysis of BST2 protein in (A) human heart lysate, (B) human ovary lysate and (C) partial recombinant BST2 protein with monoclonal BST2 antibody at a concentration of 1 ug/ml. In the tested human lysates, two distinct bands were observed which represents the glycosylated (lower bands) and the ubiquitinated (higher bands ) forms of BST2 protein.![Immunohistochemistry-Paraffin: BST2 Antibody (2E2) - BSA Free [NBP2-29621]](https://resources.bio-techne.com/images/products/BST2-Antibody-2E2-Immunohistochemistry-Paraffin-NBP2-29621-img0004.jpg "Immunohistochemistry-Paraffin: BST2 Antibody (2E2) - BSA Free [NBP2-29621]")
Immunohistochemistry-Paraffin: BST2 Antibody (2E2) - BSA Free [NBP2-29621]
Immunohistochemistry-Paraffin: BST2 Antibody (2E2) [NBP2-29621] - Analysis of BST2 protein in a section of human endometrial carcinoma using 5 ug/ml concentration of BST2 antibody (clone 2E2). The carcinoma cells depicted distinct membrane-cytoplasmic BST2 positivity with more intense staining in the cellular membranes, whereas, weak staining was observed in tumor stroma.![Immunohistochemistry-Paraffin: BST2 Antibody (2E2) - BSA Free [NBP2-29621]](https://resources.bio-techne.com/images/products/BST2-Antibody-2E2-Immunohistochemistry-Paraffin-NBP2-29621-img0002.jpg "Immunohistochemistry-Paraffin: BST2 Antibody (2E2) - BSA Free [NBP2-29621]")
Immunohistochemistry-Paraffin: BST2 Antibody (2E2) - BSA Free [NBP2-29621]
Immunohistochemistry-Paraffin: BST2 Antibody (2E2) [NBP2-29621] - Analysis of BST2 protein in a section of human renal cell carcinoma (clear cell type) using 5 ug/ml concentration of BST2 antibody (clone 2E2). The carcinoma cells depicted distinct membrane-cytoplasmic BST2 positivity with more intense staining in cellular membranes.Applications for BST2 Antibody (2E2) - BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 ug/ml
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1 ug/ml
Application Notes
The unprocessed form of BST2 protein is 180 amino acids long (161 amino acids in its cleaved BST) and its cleaved form undergoes further modifications namely disulfide bond formation, GPI-anchor/GPI-like-anchor binding, glycosylation, isopeptide bond formation, lipidation and Ubl conjugation. The predicted molecular weight of unmodified BST2 protein is ~17-18kDa, whereas the post-translationally modified protein has been documented to run at ~25-36kDa (glycosylated), 50-55 kDa (ubiquitinated) and 65-70 kDa (dimers) in WB assay. BST2 protein localize mainly to the cell membranes from where it shuttles to the trans-Golgi network, late endosome and the cytoplasm. In immuno-staining assays, BST2 may develop a membrane-cytoplasmic staining pattern. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BST-2/Tetherin
Long Name
Bone Marrow Stromal Cell Antigen 2
Alternate Names
BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin
Entrez Gene IDs
684 (Human)
Gene Symbol
BST2
UniProt
Additional BST-2/Tetherin Products
Product Documents for BST2 Antibody (2E2) - BSA Free
Product Specific Notices for BST2 Antibody (2E2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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