BST2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27154

Novus Biologicals, part of Bio-Techne
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NBP2-27154
NBP2-27154SS
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Azide Free
NBP2-45265
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse

Applications

Validated:

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all BST-2/Tetherin Antibodies »

Product Specifications

Immunogen

A portion of amino acids 80-130 of human BST2 was used as the immunogen for this antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BST2 Antibody

Western Blot: BST2 Antibody [NBP2-27154]

Western Blot: BST2 Antibody [NBP2-27154]

Western Blot: BST2 Antibody [NBP2-27154] - Analysis using BST2 antibody. Human heart in the 1) absence and 2) presence of immunizing peptide, 3) human Jurkat, 4) mouse RAW lysate and 5) recombinant human BST2 protein probed with BST2 antibody at 4 ug/mL. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Frozen: BST2 Antibody [NBP2-27154]

Immunohistochemistry-Frozen: BST2 Antibody [NBP2-27154]

Immunohistochemistry-Frozen: BST2 Antibody [NBP2-27154] - BST-2 (green) was detected in human skin (nevus) using Ang-2 antibody (1:50; 1 hour) and secondary anti-rabbit-FITC (1:500; 30 min) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.
Immunohistochemistry-Paraffin: BST2 Antibody [NBP2-27154]

Immunohistochemistry-Paraffin: BST2 Antibody [NBP2-27154]

Immunohistochemistry-Paraffin: BST2 Antibody [NBP2-27154] - Analysis of BST2 in human liver tissue using BST2 antibody at 5 ug/mL.
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Applications for BST2 Antibody

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 21695198)

Immunohistochemistry-Frozen

reported by customer review

Immunohistochemistry-Paraffin

5 ug/mL

Simple Western

1:200

Western Blot

2 - 4 ug/mL
Application Notes
BST2 is a 180 amino acid protein with an observed molecular weight of approximately 30 kDa in Western blot (see Ishikawa et all, 1995 PMID 7607676).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4 using NBP2-27154 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.1 mL PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BST-2/Tetherin

BST2, also known as CD317, HM1.24 antigen, DAMP-2 and recently designated tetherin, is an integral membrane protein with a novel topology. The specific function of the BST2, encoded by the bone marrow stromal cell antigen 2 gene, is undetermined; however, it may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, Nk cells and DCs (at protein level).

Long Name

Bone Marrow Stromal Cell Antigen 2

Alternate Names

BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin

Gene Symbol

BST2

UniProt

Q10589 (Human)

Additional BST-2/Tetherin Products

Product Documents for BST2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BST2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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