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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # M33-P5B11

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Peptide Sequence - KYQGDGIVED (amino acids 900 - 909)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for BTF Antibody (M33-P5B11) - BSA Free

Western Blot: BTF Antibody (M33-P5B11)BSA Free [NBP2-50118]

Western Blot: BTF Antibody (M33-P5B11)BSA Free [NBP2-50118]

Western Blot: BTF Antibody (M33-P5B11) [NBP2-50118] - Western Blot using mAb M33-P5B11.
Immunohistochemistry: BTF Antibody (M33-P5B11) - BSA Free [NBP2-50118]

Immunohistochemistry: BTF Antibody (M33-P5B11) - BSA Free [NBP2-50118]

Immunohistochemistry: BTF Antibody (M33-P5B11) [NBP2-50118] - IHC was performed on formalin-fixed, paraffin-embedded tissue sections using Anti-BCLAF1 [M33-P5B11]. Image is showing both cytoplasmic and nuclear localisation positivity of BCLAF1 in colon cancer which is in agreement with existing literature. Exclusively nuclear localisation of BCLAF1 is observed in non-neoplastic colon mucosa. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).

Applications for BTF Antibody (M33-P5B11) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): ELISA- Peptide immunogen; Western Blot- Hela whole cell extract; IHC- Human colon carcinoma.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BTF

Btf localization is regulated by apoptotic signals, and loss of emerin binding to Btf may be relevant to muscle wasting in Emery-Dreifuss muscular dystrophy.

Alternate Names

bcl-2-associated transcription factor 1, BCL2-associated transcription factor 1, Btf, BTFKIAA0164bK211L9.1

Gene Symbol

BCLAF1

Additional BTF Products

Product Documents for BTF Antibody (M33-P5B11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BTF Antibody (M33-P5B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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