BTF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89924

Novus Biologicals, part of Bio-Techne
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NBP1-89924
NBP1-89924-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BTF Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QKSPEIHRRIDISPSTLRKHTRLAGEERVFKEENQKGDKKLRCDSADLRHDIDRRRKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKSYKDDSKHKREQDHSRSSSSSASPSSPSSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BTF Antibody

Western Blot: BTF Antibody [NBP1-89924]

Western Blot: BTF Antibody [NBP1-89924]

Western Blot: BTF Antibody [NBP1-89924] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: BTF Antibody [NBP1-89924]

Immunocytochemistry/ Immunofluorescence: BTF Antibody [NBP1-89924]

Immunocytochemistry/Immunofluorescence: BTF Antibody [NBP1-89924] - Staining of human cell line A-431 shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]

Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924]

Immunohistochemistry-Paraffin: BTF Antibody [NBP1-89924] - Staining of human colon.
View 7 more images

Applications for BTF Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BTF

Btf localization is regulated by apoptotic signals, and loss of emerin binding to Btf may be relevant to muscle wasting in Emery-Dreifuss muscular dystrophy.

Alternate Names

bcl-2-associated transcription factor 1, BCL2-associated transcription factor 1, Btf, BTFKIAA0164bK211L9.1

Gene Symbol

BCLAF1

Additional BTF Products

Product Documents for BTF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BTF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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