BTK Antibody (2J9O8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15612

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15612-20ul
NBP3-15612-100ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2J9O8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BTK Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 560-659 of human BTK (Q06187). PVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BTK Antibody (2J9O8)

Western Blot: BTK Antibody (2J9O8) [NBP3-15612]

Western Blot: BTK Antibody (2J9O8) [NBP3-15612]

Western Blot: BTK Antibody (2J9O8) [NBP3-15612] - Western blot analysis of extracts of various cell lines, using BTK antibody (NBP3-15612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry: BTK Antibody (2J9O8) [NBP3-15612]

Immunohistochemistry: BTK Antibody (2J9O8) [NBP3-15612]

Immunohistochemistry: BTK Antibody (2J9O8) [NBP3-15612] - Immunofluorescence analysis of mouse spleen using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612]

Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612]

Immunohistochemistry-Paraffin: BTK Antibody (2J9O8) [NBP3-15612] - Immunohistochemistry of paraffin-embedded human lung cancer using BTK Rabbit mAb (NBP3-15612) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for BTK Antibody (2J9O8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BTK

Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. All members of the BTK/Tec family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Long Name

Bruton Agammaglobulinemia Tyrosine Kinase

Alternate Names

AGMX1, IMD1, PSCTK1, XLA

Gene Symbol

BTK

Additional BTK Products

Product Documents for BTK Antibody (2J9O8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for BTK Antibody (2J9O8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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