BubR1 Antibody (7H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15207

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15207
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7H4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all BubR1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human BubR1. UniProt ID (O60566).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BubR1 Antibody (7H4)

Western Blot: BubR1 Antibody (7H4) [NBP3-15207]

Western Blot: BubR1 Antibody (7H4) [NBP3-15207]

Western Blot: BubR1 Antibody (7H4) [NBP3-15207] - Positive WB detected in: K562 whole cell lysate, U-251 whole cell lysate. All lanes: BubR1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 120, 106, 122 KDa. Observed band size: 125 KDa.
Flow Cytometry: BubR1 Antibody (7H4) [NBP3-15207]

Flow Cytometry: BubR1 Antibody (7H4) [NBP3-15207]

Flow Cytometry: BubR1 Antibody (7H4) [NBP3-15207] - Overlay histogram showing Hela cells stained with BubR1 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for BubR1 Antibody (7H4)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: BubR1

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.

BubR1 antibodies are useful tools for cancer research and cell division studies.

Alternate Names

Bub1A, BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta, budding uninhibited by benzimidazoles 1 homolog beta (yeast), EC 2.7.11.1, hBUBR1, MAD3/BUB1-related protein kinase, MAD3LBUB1beta, Mitotic checkpoint kinase MAD3L, Protein SSK1, SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Gene Symbol

BUB1B

Additional BubR1 Products

Product Documents for BubR1 Antibody (7H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BubR1 Antibody (7H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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