BubR1 Antibody (OTI6E5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47680

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6E5

Concentration

1 mg/ml

View all BubR1 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human BUB1B expression vector

Specificity

This antibody is specific for Homo sapiens budding uninhibited by benzimidazoles 1 homolog beta (yeast) (BUB1B).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

119.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BubR1 Antibody (OTI6E5)

Western Blot: BubR1 Antibody (OTI6E5) [NBP1-47680]

Western Blot: BubR1 Antibody (OTI6E5) [NBP1-47680]

Western Blot: BubR1 Antibody (6E5) [NBP1-47680] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.
Immunocytochemistry/ Immunofluorescence: BubR1 Antibody (OTI6E5) [NBP1-47680]

Immunocytochemistry/ Immunofluorescence: BubR1 Antibody (OTI6E5) [NBP1-47680]

Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) [NBP1-47680] - BubR1 Antibody (6E5) [NBP1-47680] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.
Immunohistochemistry-Paraffin: BubR1 Antibody (OTI6E5) [NBP1-47680]

Immunohistochemistry-Paraffin: BubR1 Antibody (OTI6E5) [NBP1-47680]

Immunohistochemistry-Paraffin: BubR1 Antibody (6E5) [NBP1-47680] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.
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Applications for BubR1 Antibody (OTI6E5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:200-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BubR1

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome(APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Alternate Names

Bub1A, BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta, budding uninhibited by benzimidazoles 1 homolog beta (yeast), EC 2.7.11.1, hBUBR1, MAD3/BUB1-related protein kinase, MAD3LBUB1beta, Mitotic checkpoint kinase MAD3L, Protein SSK1, SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Entrez Gene IDs

701 (Human)

Gene Symbol

BUB1B

Additional BubR1 Products

Product Documents for BubR1 Antibody (OTI6E5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BubR1 Antibody (OTI6E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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