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BUD13 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81863

Novus Biologicals, part of Bio-Techne
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NBP1-81863
NBP1-81863-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for BUD13 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PGHQDSDSDLSPPRNRPRHRSSDSDLSPPRRRQRTKSSDSDLSPPRRSQPPGKKAAHMYSGAKTGLVLTDIQREQQELKEQDQETM

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BUD13 Antibody

Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863]

Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863]

Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human cerebral cortex, colon, kidney and testis using Anti-BUD13 antibody NBP1-81863 (A) shows similar protein distribution across tissues to independent antibody NBP2-38407 (B).
Western Blot: BUD13 Antibody [NBP1-81863]

Western Blot: BUD13 Antibody [NBP1-81863]

Western Blot: BUD13 Antibody [NBP1-81863] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: BUD13 Antibody [NBP1-81863]

Immunocytochemistry/ Immunofluorescence: BUD13 Antibody [NBP1-81863]

Immunocytochemistry/Immunofluorescence: BUD13 Antibody [NBP1-81863] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Applications for BUD13 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BUD13

Alternate Names

BUD13 homolog (S. cerevisiae), fSAP71, MGC13125

Gene Symbol

BUD13

Additional BUD13 Products

Product Documents for BUD13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BUD13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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