BUD13 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81863
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for BUD13 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PGHQDSDSDLSPPRNRPRHRSSDSDLSPPRRRQRTKSSDSDLSPPRRSQPPGKKAAHMYSGAKTGLVLTDIQREQQELKEQDQETM
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BUD13 Antibody
Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863]
Immunohistochemistry-Paraffin: BUD13 Antibody [NBP1-81863] - Staining of human cerebral cortex, colon, kidney and testis using Anti-BUD13 antibody NBP1-81863 (A) shows similar protein distribution across tissues to independent antibody NBP2-38407 (B).Western Blot: BUD13 Antibody [NBP1-81863]
Western Blot: BUD13 Antibody [NBP1-81863] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/ Immunofluorescence: BUD13 Antibody [NBP1-81863]
Immunocytochemistry/Immunofluorescence: BUD13 Antibody [NBP1-81863] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.Applications for BUD13 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BUD13
Alternate Names
BUD13 homolog (S. cerevisiae), fSAP71, MGC13125
Gene Symbol
BUD13
Additional BUD13 Products
Product Documents for BUD13 Antibody
Product Specific Notices for BUD13 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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