Butyrylcholinesterase/BCHE Antibody (OTI1E1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03523

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Canine

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E1

Concentration

0.72 mg/ml

View all Butyrylcholinesterase/BCHE Antibodies »

Product Summary for Butyrylcholinesterase/BCHE Antibody (OTI1E1)

Immunogen

Full length human recombinant protein of human BCHE (NP_000046) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Butyrylcholinesterase/BCHE Antibody (OTI1E1)

Western Blot: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523]

Western Blot: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523]

Western Blot: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Butyrylcholinesterase monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Flow Cytometry: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523]

Flow Cytometry: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523]

Flow Cytometry: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Butyrylcholinesterase antibody, and then analyzed by flow cytometry.
Western Blot: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523]

Western Blot: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523]

Western Blot: Butyrylcholinesterase/BCHE Antibody (OTI1E1) [NBP2-03523] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Butyrylcholinesterase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Butyrylcholinesterase.

Applications for Butyrylcholinesterase/BCHE Antibody (OTI1E1)

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.72 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Butyrylcholinesterase/BCHE

Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq].

Alternate Names

Acylcholine acylhydrolase, BCHE, Butyrylcholine esterase, CHE1, Pseudocholinesterase

Entrez Gene IDs

590 (Human)

Gene Symbol

BCHE

Additional Butyrylcholinesterase/BCHE Products

Product Documents for Butyrylcholinesterase/BCHE Antibody (OTI1E1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Butyrylcholinesterase/BCHE Antibody (OTI1E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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