We have a customer who is interested in the following antibodies: NB600-904 c-Fos ; NBP1-02935 ATF3. She is not sure which ones would be suitable for her studies. Her message is as follows: I have a new list of antibodies I would like you to check for me. We need them for paraformaldehyde perfused mice, the DRGs (dorsal root ganglia) cut by frozen section. So not paraffin processed. Would Novus be able to comment on the suitability of these antibodies for our tissues. I would also be grateful if they could make other suggestions if they have other more suitable antibodies or comment on which of the two Il1b antibodies would be the better. I am hoping you are able to help us with our customer.

While NBP1-02935 has not been tested on frozen sections, none of our other ATF3 antibodies have been tested either. NBP1-02935 works fine in IHC on paraffin embedded tissues, so should be suitable for use on frozen sections as well, although we can not guarantee that. Generally an antibody that is suitable for paraffin-embedded sections should work on frozen sections as well, however, we have seen evidence that this is not always true. This is why we make a distinction. The customer is more than welcome to try an IHC-P guaranteed antibody on frozen sections if they would like since it will most likely work, however, we can not guarantee it for this use. NB110-61652 is sheep polyclonal, not NB600-904 and once again, NB600-904 isn't guaranteed for use in frozen sections but the customer can certainly give it a try. I recommended NB110-75039 since it does have a frozen tissue guarantee. NB110-75039 and NB110-61652 are both guaranteed to detect c-Fos on frozen sections. I would recommend NB110-75039 since we do have images for this product and it is a rabbit polyclonal, so it is much more likely the customer would have an anti-rabbit secondary available rather than an anti-sheep secondary.

I am looking for some c-fos antibodies that could work in IHC.

Here are a list of products which might be of interest to you: Product List. We have additional filters on the left which may help you narrow down based on host species, target species, clonality, etc. We will guarantee these to work for all stated species and applications listed on the datasheet.

c-Fos Antibody

Name: c-Fos Antibody
Brand: Novus Biologicals
SKU: NB600-904

Novus Biologicals, part of Bio-Techne | Catalog # NB600-904

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all c-Fos Antibodies »

Product Summary for c-Fos Antibody

Immunogen

This c-Fos Antibody was developed against synthetic C-Fos peptide (Human, C-terminal).

Reactivity Notes

Not yet tested in other species.

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for c-Fos Antibody

Western Blot: c-Fos Antibody [NB600-904]

Western Blot: c-Fos Antibody [NB600-904] - Aanalysis of c-Fos p62 expression in A431 (A) and HeLa (B) whole cell lysates.

Applications for c-Fos Antibody

Application

Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:200

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 0.2% gelatin

Preservative

0.1% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: c-Fos

c-Fos is an intermediate early gene that is one of four members of the FOS family of activator protein-1 (AP-1) transcription factors, which also includes Fra-1, Fra-2, and FosB (1-3). Under the FOS gene, human c-Fos is synthesized as a protein of 308 amino acids (aa) with a basic leucine zipper (bZip) domain important for dimerization, and a basic domain for interacting with DNA, with a theoretical molecular weight of ~40.6 kDa (4,5). c-Fos can heterodimerize with members of the JUN family (c-Jun, JunB, and JunD) to form the active transcription factor AP-1 complex (3,5). AP-1 related proteins bind TPA-related responsive elements (TRE), cAMP responsive elements (CRE), and related sequences, with c-Fos:c-Jun dimers partial to TRE sites (5).

In response to stimuli, c-Fos, which is encoded by protooncogenes, has a role in cell proliferation, differentiation, and transformation (3,6). A variety of stimuli can increase c-Fos expression such as growth factors, proinflammatory cytokines, UV radiation, neurotransmitters, hormones, injury, and stress (1,6). c-Fos has long been used as a marker for neuronal activity and is associated with neural and behavioral responses following stimuli (1-3, 6-7). Mouse studies have revealed that c-Fos is important for efficient neurogenesis and cortical development (3). Additionally, c-Fos signal can be used as a molecular marker for learning and memory, such as recognition and fear (2,7). Studies have found that repeated positive stimuli result in increased Fos expression while, conversely, repeated negative value stimuli are indicated by decreased signal (7). Intermediate early genes have also been implicated in neuropsychiatric disorders including showing altered c-Fos expression in a schizophrenia animal model (2). Furthermore, antipsychotics and antidepressants are both capable of impacting c-Fos expression (2).

References

1. Kovacs K. J. (1998). c-Fos as a transcription factor: a stressful (re)view from a functional map. Neurochemistry International. https://doi.org/10.1016/s0197-0186(98)00023-0

2. Gallo, F. T., Katche, C., Morici, J. F., Medina, J. H., & Weisstaub, N. V. (2018). Immediate Early Genes, Memory and Psychiatric Disorders: Focus on c-Fos, Egr1 and Arc. Frontiers in Behavioral Neuroscience. https://doi.org/10.3389/fnbeh.2018.00079

3. Velazquez, F. N., Caputto, B. L., & Boussin, F. D. (2015). c-Fos importance for brain development. Aging. https://doi.org/10.18632/aging.100862

4. Uniprot (P01100)

5. Wu, Z., Nicoll, M., & Ingham, R. J. (2021). AP-1 family transcription factors: a diverse family of proteins that regulate varied cellular activities in classical hodgkin lymphoma and ALK+ ALCL. Experimental Hematology & Oncology. https://doi.org/10.1186/s40164-020-00197-9

6. Shaulian, E., & Karin, M. (2001). AP-1 in cell proliferation and survival. Oncogene. https://doi.org/10.1038/sj.onc.1204383

7. Chung L. (2015). A Brief Introduction to the Transduction of Neural Activity into Fos Signal. Development & Reproduction. https://doi.org/10.12717/DR.2015.19.2.061

Long Name

FBJ/Finkel–Biskis–Jinkins Murine Osteosarcoma Viral Oncogene Homolog

Alternate Names

cFos, FOS, G0S7

Entrez Gene IDs

2353 (Human); 14281 (Mouse); 314322 (Rat)

Gene Symbol

FOS

UniProt

P01100 (Human)

Additional c-Fos Products

Product Documents for c-Fos Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for c-Fos Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

c-Fos Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-904

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Concentration

View all c-Fos Antibodies »

Product Summary for c-Fos Antibody

Immunogen

Reactivity Notes

Localization

Clonality

Host

Isotype

Scientific Data Images for c-Fos Antibody

Western Blot: c-Fos Antibody [NB600-904]

Applications for c-Fos Antibody

Immunohistochemistry

Immunohistochemistry-Paraffin

Immunoprecipitation

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: c-Fos

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional c-Fos Products

Product Documents for c-Fos Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for c-Fos Antibody