c-Maf Antibody (JE39-57)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32197
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE39-57
Concentration
1 mg/ml
Product Summary for c-Maf Antibody (JE39-57)
Immunogen
Synthetic peptide within Human c-Maf aa 324-373 / 373. (Uniprot: O75444)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for c-Maf Antibody (JE39-57)
Western Blot: c-Maf Antibody (JE39-57) [NBP3-32197]
Western Blot: c-Maf Antibody (JE39-57) [NBP3-32197] - Western blot analysis of c-Maf on different lysates with Rabbit anti-c-Maf antibody (NBP3-32197) at 1/1,000 dilution.Lane 1: A549 cell lysate (10 ug/Lane)
Lane 2: Raji cell lysate (10 ug/Lane)
Lane 3: Mouse lung tissue lysate (20 ug/Lane)
Lane 4: Mouse kidney tissue lysate (20 ug/Lane)
Lane 5: Rat spleen tissue lysate (20 ug/Lane)
Lane 6: Rat kidney tissue lysate (20 ug/Lane)
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32197) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for c-Maf Antibody (JE39-57)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: c-Maf
Long Name
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian)
Alternate Names
CCA4, cMaf, MAF2
Gene Symbol
MAF
Additional c-Maf Products
Product Documents for c-Maf Antibody (JE39-57)
Product Specific Notices for c-Maf Antibody (JE39-57)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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