c-Maf Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24551

Novus Biologicals, part of Bio-Techne
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NBP2-24551
NBP2-24551SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine

Cited:

Human, Mouse

Predicted:

Monkey (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all c-Maf Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 75-110 of human c-maf was used as the immunogen, GenBank no. NP_001026974.1|.

Reactivity Notes

Chimpanzee (87%), Bovine, Equine (81%).

Specificity

100% homologous in human isoforms CRA_a, CRA_b, and CRA_c).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for c-Maf Antibody - BSA Free

Western Blot: c-Maf AntibodyBSA Free [NBP2-24551]

Western Blot: c-Maf AntibodyBSA Free [NBP2-24551]

Western Blot: c-Maf Antibody [NBP2-24551] - Total protein from Human Pancreas and Human Small Intestine was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-cMaf in 1% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Western Blot: c-Maf AntibodyBSA Free [NBP2-24551]

Western Blot: c-Maf AntibodyBSA Free [NBP2-24551]

Western Blot: c-Maf Antibody [NBP2-24551] - SUM159 and HCC1954 whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. c-Maf Antibody (NBP2-24551) was used for primary antibody: 1:500, 4C, overnight. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: c-Maf Antibody - BSA Free [NBP2-24551]

Immunohistochemistry-Paraffin: c-Maf Antibody - BSA Free [NBP2-24551]

Immunohistochemistry-Paraffin: c-Maf Antibody [NBP2-24551] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human liver cancer using c-MAF antibody at 2ug/ml concentration (1:500 dilution). The primary antibody binding to c-MAF protein was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This c-MAF antibody showed a very strong nuclear and moderate to strong staining in the endothelial cells (blood vessels) and in a subset of apparently infiltrating inflammatory cells. Weak to moderate cytoplasmic and nuclear staining was observed in liver cancer cells and the cells of tumor stroma including fibroblasts.
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Applications for c-Maf Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

2-4 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 3.3 using NBP2-24551 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1 ml PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: c-Maf

c-Maf is part of the Maf family of transcription factors that possess a conserved basic region and a leucine zipper domain that mediate DNA and protein-protein binding and plays an important role in tissue-specific gene regulation and cell differentiation. c-Maf is a transcriptional activator of interleukin 4 and 10 in T-cells, and is involved in lens fiber cell differentiation. c-Maf translocation has been observed in human multiple myeloma and may be oncogenic.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (Avian)

Alternate Names

CCA4, cMaf, MAF2

Gene Symbol

MAF

UniProt

O75444

Additional c-Maf Products

Product Documents for c-Maf Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for c-Maf Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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