C-Peptide Antibody (2B7)
Novus Biologicals, part of Bio-Techne | Catalog # NB110-2555
Key Product Details
Species Reactivity
Human, Bovine (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative)
Applications
Competitive ELISA, ELISA, Radioimmunoassay, Radioimmunodiffusion
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2B7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Hybridoma clone has been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with recombinant C-peptide construction.
Epitope
The three-dimensional structure generated by N- and C- terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.
Reactivity Notes
Cross-reacts with Human. Does not react with Bovine, Mouse, Rat or Pig
Localization
Secreted
Specificity
Affinity (Kd) > 1 x 10^8. Binds to the human free C-peptide and C-peptide region in proinsulin molecules. Antigenic determinants identified by MAbs are in the three-dimensional structure generated by N- and C- terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.. No cross-reactivity with human, bovine, porcine and mouse/rat insulin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Applications for C-Peptide Antibody (2B7)
Application
Recommended Usage
ELISA
1:100-1:2000
Radioimmunoassay
1:100-1:2000
Application Notes
Competitive ELISA or RIA detection of C-peptide in human serum without or after immunospecific removal of proinsulin (with solid-phased ab8299 (1D6) to human proinsulin)Construction of a sensitive two-site sandwich EIA/ RIA immunosorbent assays for the measurement of total (including proinsulin convertion intermediates) human proinsulin in serum. ab8297 used in conjunction with this antibody would be suitable.Specific immunosorbtional (with solid-phase 2B7) removal of total proinsulin and C-peptide molecules from serum samples before the measurement of human insulin
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: C-Peptide
Long Name
Proinsulin C-Peptide
Alternate Names
C-P, Insulin C-Peptide, Proinsulin C-Peptide
Gene Symbol
INS
Additional C-Peptide Products
Product Documents for C-Peptide Antibody (2B7)
Product Specific Notices for C-Peptide Antibody (2B7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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