C-Peptide Antibody (CII-29)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05439

Novus Biologicals, part of Bio-Techne
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NBP1-05439
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

ELISA, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CII-29

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all C-Peptide Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragments of rat C-peptides I and II conjugated with the carrier protein.

Specificity

Reacts with rat c-peptide I, rat c-peptide II, mouse c-peptide I, mouse c-peptide II and rat proinsulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for C-Peptide Antibody (CII-29)

Application
Recommended Usage

ELISA

1:100-1:2000

Sandwich ELISA

1:100-1:2000
Application Notes
Can be used for separate or consistent detection of rat C-peptide isoforms (rat C-peptide I and/or rat C-peptide II) in biological samples. The C-Peptide Antibody (CC27) NBP1-05434 can be use as the capture antibody with the use of detection antibody C-Peptide Antibody (CII-29) NBP1-05439 in Sandwich ELISA. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 28988715). Use in IP reported in secitific publication PMID: 32433667

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: C-Peptide

C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Long Name

Proinsulin C-Peptide

Alternate Names

C-P, Insulin C-Peptide, Proinsulin C-Peptide

Entrez Gene IDs

16334 (Mouse); 24506 (Rat)

Gene Symbol

INS

Additional C-Peptide Products

Product Documents for C-Peptide Antibody (CII-29)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for C-Peptide Antibody (CII-29)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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