C12orf73 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90536
![Western Blot: C12orf73 Antibody [NBP1-90536]](https://resources.bio-techne.com/images/products/C12orf73-Antibody-Western-Blot-NBP1-90536-img0014.jpg "Western Blot: C12orf73 Antibody [NBP1-90536]")
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Zebrafish
Cited:
Fish - Danio rerio (Zebrafish)
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Knockout Validated, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AEVVHRYYRPDLTIPEIPPKRGELKTELLGLKERKHKPQVSQQEEL
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%). Zebrafish reactivity reported in (PMID: 32161263).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for C12orf73 Antibody
![Western Blot: C12orf73 Antibody [NBP1-90536]](https://resources.bio-techne.com/images/products/C12orf73-Antibody-Western-Blot-NBP1-90536-img0013.jpg "Western Blot: C12orf73 Antibody [NBP1-90536]")
Western Blot: C12orf73 Antibody [NBP1-90536]
Western Blot: C12orf73 Antibody [NBP1-90536] - BRAWNIN (C12orf73; BR) is a conserved SEP at the inner mitochondrial membrane. Endogenous BR is detected in HeLa and HEK293T with an alpha-BR antibody. OE = overexpression. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-020-14999-2), licensed under a CC-BY license.![Immunocytochemistry/ Immunofluorescence: C12orf73 Antibody [NBP1-90536]](https://resources.bio-techne.com/images/products/C12orf73-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90536-img0010.jpg "Immunocytochemistry/ Immunofluorescence: C12orf73 Antibody [NBP1-90536]")
Immunocytochemistry/ Immunofluorescence: C12orf73 Antibody [NBP1-90536]
Immunocytochemistry/Immunofluorescence: C12orf73 Antibody [NBP1-90536] - Endogenous BR (C12orf73) detected by alpha-BR in HeLa costained with mitotracker. Scale = 5 uM. h Endogenous BR detected by alpha-BR in HEK293T costained with mitotracker. Scale = 5 uM. i alpha-BR and alpha-citrate synthase (CS) immunofluorescence in mouse skeletal muscle, longitudinal section. Scale = 20 uM. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-020-14999-2/figures/2), licensed under a CC-BY license.![Immunohistochemistry-Paraffin: C12orf73 Antibody [NBP1-90536]](https://resources.bio-techne.com/images/products/C12orf73-Antibody-Immunohistochemistry-Paraffin-NBP1-90536-img0009.jpg "Immunohistochemistry-Paraffin: C12orf73 Antibody [NBP1-90536]")
Immunohistochemistry-Paraffin: C12orf73 Antibody [NBP1-90536]
Immunohistochemistry-Paraffin: C12orf73 Antibody [NBP1-90536] - Staining of human cerebral cortex shows strong positivity in neuropil.![Immunocytochemistry/ Immunofluorescence: C12orf73 Antibody [NBP1-90536]](https://resources.bio-techne.com/images/products/C12orf73-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90536-img0004.jpg "Immunocytochemistry/ Immunofluorescence: C12orf73 Antibody [NBP1-90536]")
![Immunohistochemistry-Paraffin: C12orf73 Antibody [NBP1-90536]](https://resources.bio-techne.com/images/products/C12orf73-Antibody-Immunohistochemistry-Paraffin-NBP1-90536-img0006.jpg "Immunohistochemistry-Paraffin: C12orf73 Antibody [NBP1-90536]")
![Immunohistochemistry-Paraffin: C12orf73 Antibody [NBP1-90536]](https://resources.bio-techne.com/images/products/C12orf73-Antibody-Immunohistochemistry-Paraffin-NBP1-90536-img0007.jpg "Immunohistochemistry-Paraffin: C12orf73 Antibody [NBP1-90536]")
![Immunohistochemistry-Paraffin: C12orf73 Antibody [NBP1-90536]](https://resources.bio-techne.com/images/products/C12orf73-Antibody-Immunohistochemistry-Paraffin-NBP1-90536-img0008.jpg "Immunohistochemistry-Paraffin: C12orf73 Antibody [NBP1-90536]")
![Knockdown Validated: C12orf73 Antibody [NBP1-90536]](https://resources.bio-techne.com/images/products/C12orf73-Antibody-Knockdown-Validated-NBP1-90536-img0011.jpg "Western Blot: C12orf73 Antibody [NBP1-90536]")
![Knockout Validated: C12orf73 Antibody [NBP1-90536]](https://resources.bio-techne.com/images/products/C12orf73-Antibody-Knockout-Validated-NBP1-90536-img0012.jpg "Western Blot: C12orf73 Antibody [NBP1-90536]")
Applications for C12orf73 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200-1:500
Western Blot
Reported in scientific literature (PMID:32161263)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: C12orf73
Alternate Names
BR, BRAWNIN, chromosome 12 open reading frame 73, FLJ13975, protein BRAWNIN, uncharacterized protein C12orf73
Gene Symbol
C12ORF73
Additional C12orf73 Products
Product Documents for C12orf73 Antibody
Product Specific Notices for C12orf73 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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