C16orf72 Antibody (OTI2D1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02850
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2D1
Concentration
0.65 mg/ml
Product Summary for C16orf72 Antibody (OTI2D1)
Immunogen
Full length human recombinant protein of human C16orf72 (NP_054836) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
30.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for C16orf72 Antibody (OTI2D1)
Western Blot: C16orf72 Antibody (OTI2D1) [NBP2-02850]
Western Blot: C16orf72 Antibody (2D1) [NBP2-02850] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C16orf72 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C16orf72.
Immunocytochemistry/Immunofluorescence: C16orf72 Antibody (2D1) [NBP2-02850] Staining of COS7 cells transiently transfected by pCMV6-ENTRY C16orf72.
Flow Cytometry: C16orf72 Antibody (OTI2D1) [NBP2-02850]
Flow Cytometry: C16orf72 Antibody (2D1) [NBP2-02850] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-C16orf72 antibody, and then analyzed by flow cytometry.Applications for C16orf72 Antibody (OTI2D1)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.65 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: C16orf72
Alternate Names
chromosome 16 open reading frame 72, FLJ41272, hypothetical protein LOC29035, PRO0149
Entrez Gene IDs
29035 (Human)
Gene Symbol
C16orf72
Additional C16orf72 Products
Product Documents for C16orf72 Antibody (OTI2D1)
Product Specific Notices for C16orf72 Antibody (OTI2D1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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