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C1orf52 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93751

Novus Biologicals, part of Bio-Techne
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NBP1-93751

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PEEPPKEFKIWKSNYVPPPETYTTEKKPPPPELDMAIKWSNIYEDNGDDAPQNAKKARLLPEGEETLESDDEKDEHTSKKRKVEPGEPAKKK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for C1orf52 Antibody

Western Blot: C1orf52 Antibody [NBP1-93751]

Western Blot: C1orf52 Antibody [NBP1-93751]

Western Blot: C1orf52 Antibody [NBP1-93751] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: C1orf52 Antibody [NBP1-93751]

Immunocytochemistry/ Immunofluorescence: C1orf52 Antibody [NBP1-93751]

Immunocytochemistry/Immunofluorescence: C1orf52 Antibody [NBP1-93751] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: C1orf52 Antibody [NBP1-93751]

Immunohistochemistry-Paraffin: C1orf52 Antibody [NBP1-93751]

Immunohistochemistry-Paraffin: C1orf52 Antibody [NBP1-93751] - Staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.

Applications for C1orf52 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: C1orf52

Alternate Names

BAG, chromosome 1 open reading frame 52, FLJ44982, gm117, RP11-234D19.1

Gene Symbol

C1orf52

Additional C1orf52 Products

Product Documents for C1orf52 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for C1orf52 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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