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C1orf74 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83571

Novus Biologicals, part of Bio-Techne
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NBP1-83571
NBP1-83571-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLGYPVPYTFHLNQGDDNCLALTPLRVFTARISWLLGQPPILLYSFSVPESLFPGLRDILNTWEKDLRTRFRTQNDFADL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for C1orf74 Antibody

Western Blot: C1orf74 Antibody [NBP1-83571]

Western Blot: C1orf74 Antibody [NBP1-83571]

Western Blot: C1orf74 Antibody [NBP1-83571] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: C1orf74 Antibody [NBP1-83571]

Immunocytochemistry/ Immunofluorescence: C1orf74 Antibody [NBP1-83571]

Immunocytochemistry/Immunofluorescence: C1orf74 Antibody [NBP1-83571] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: C1orf74 Antibody [NBP1-83571]

Immunohistochemistry-Paraffin: C1orf74 Antibody [NBP1-83571]

Immunohistochemistry-Paraffin: C1orf74 Antibody [NBP1-83571] - Staining of human hippocampus shows strong cytoplasmic positivity in astrocytes.

Applications for C1orf74 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: C1orf74

Alternate Names

chromosome 1 open reading frame 74

Gene Symbol

C1ORF74

Additional C1orf74 Products

Product Documents for C1orf74 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for C1orf74 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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