C4.4A/LYPD3 Antibody (PS00-59)

Western Blot: C4.4A/LYPD3 Antibody (PS00-59) [NBP3-32550]

Western Blot: C4.4A/LYPD3 Antibody (PS00-59) [NBP3-32550] - Western blot analysis of C4.4A/LYPD3 on different lysates with Rabbit anti-C4.4A/LYPD3 antibody (NBP3-32550) at 1/1,000 dilution.

Lane 1: SK-Br-3 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: A431 cell lysate

Lysates/proteins at 10 ug/Lane.

Predicted band size: 36 kDa
Observed band size: 90 kDa

Exposure time: 1 minute 17 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32550) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: C4.4A/LYPD3 Antibody (PS00-59) [NBP3-32550]

Immunohistochemistry: C4.4A/LYPD3 Antibody (PS00-59) [NBP3-32550] - Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Rabbit anti-C4.4A/LYPD3 antibody (NBP3-32550) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32550) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.