CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54585
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # CA19.9/1390R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified human CA19-9/Sialyl Lewis A protein
Localization
Cytoplasmic
Specificity
CA19-9, a carbohydrate epitope expressed on a high MW (400kDa) mucin glycoprotein, is a sialyl Lewisa structure which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lewisa and/or Lewisb blood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductal epithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine, and pancreas. Preoperative elevated CA19-9 levels in patients with stage I pancreatic carcinoma decrease to normal values following surgery. When used serially, CA19-9 can predict recurrence of disease prior to radiographic or clinical findings. This monoclonal antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.
Marker
GI Tumor Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
400 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified by Protein A column. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53220).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) - Azide and BSA Free
Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) - Azide and BSA Free [NBP2-54585]
Immunohistochemistry-Paraffin: CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) - Azide and BSA Free [NBP2-54585] - Human Gastric Carcinoma stained with CA19-9 Recombinant Rabbit Monoclonal Antibody (CA19.9/1390R).Applications for CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 5-10ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CA19-9
Long Name
Carbohydrate Antigen 19-9
Alternate Names
CA 19-9
Additional CA19-9 Products
Product Documents for CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) - Azide and BSA Free
Product Specific Notices for CA19-9/Sialyl Lewis A Antibody (CA19.9/1390R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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