CABP1 Antibody (JE56-72)

Western Blot: CABP1 Antibody (JE56-72) [NBP3-32099]

Western Blot: CABP1 Antibody (JE56-72) [NBP3-32099] - Western blot analysis of CABP1 on different lysates with Rabbit anti-CABP1 antibody (NBP3-32099) at 1/1,000 dilution.

Lane 1: U-87 MG cell lysate (20 ug/Lane)
Lane 2: Human brain tissue lysate (40 ug/Lane)

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32099) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: CABP1 Antibody (JE56-72) [NBP3-32099]

Flow Cytometry: CABP1 Antibody (JE56-72) [NBP3-32099] - Flow cytometric analysis of U-87 MG cells labeling CABP1.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32099, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).