Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S178A-9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Summary for CACNA1G Antibody (S178A-9)

Immunogen

Fusion protein amino acids 2052-2172 (cytoplasmic C-terminus) of mouse Cav3.1

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Membrane

Specificity

Detects

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CACNA1G Antibody (S178A-9)

Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322]

Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322]

Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322] - Western Blot analysis of Rat brain membrane lysate showing detection of CACNA1G Calcium Channel protein using Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Primary Antibody: Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody (NBP2-59322) at 1:1000.
Immunocytochemistry/ Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322]

Immunocytochemistry/ Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322]

Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) CACNA1G Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322]

Immunocytochemistry/ Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322]

Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X.
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Applications for CACNA1G Antibody (S178A-9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NBP2-59322 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: CACNA1G

Voltage-activated calcium channels can be distinguished based on their voltage-dependence, deactivation, and single-channel conductance. See MIM 601011. Low-voltage-activated calcium channels are referred to as 'T' type because their currents are both transient, owing to fast inactivation, and tiny, owing to small conductance. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing.(supplied by OMIM)

Long Name

Voltage-dependent T-type calcium channel subunit alpha-1G

Alternate Names

Cav3.1c, KIAA1123, NBR13

Gene Symbol

CACNA1G

Additional CACNA1G Products

Product Documents for CACNA1G Antibody (S178A-9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CACNA1G Antibody (S178A-9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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