CACNA2D1 Antibody (20A)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-2864

Novus Biologicals, part of Bio-Techne
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NB120-2864-0.025ml
NB120-2864
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Guinea Pig, Rabbit

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 20A

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CACNA2D1 Antibodies »

Product Specifications

Immunogen

Purified rabbit dihydropyridine receptor.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 16938495). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Detects 1,4-dihydropyridine (DHP) receptor alpha-2 subunit from skeletal and cardiac muscle. Detects a band at 220 kDa under non-reducing and a 143 kDa band under reducing conditions representing DHP from rabbit skeletal muscle membrane preparations. Immunohistochemical staining of DHP in rabbit skeletal muscle with NB120-2864 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CACNA2D1 Antibody (20A)

Western Blot: CACNA2D1 Antibody (20A) [NB120-2864]

Western Blot: CACNA2D1 Antibody (20A) [NB120-2864]

Western Blot: CACNA2D1 Antibody (20A) [NB120-2864] - Primary antibody dilution- 1:1000 for 2 Hours at RT, in blocking buffer, 5% Milk in TBS-0.05% Tween : Blocking Overnight at +4 degree C. Secondary antibody : 1:2000 in blocking buffer, 1 hour at RT. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864]

Immunocytochemistry/ Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864]

Immunocytochemistry/Immunofluorescence: CACNA2D1 Antibody (20A) [NB120-2864] - Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Dihydropyridine Receptor alpha-2 monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864]

Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864]

Immunohistochemistry-Paraffin: CACNA2D1 Antibody (20A) [NB120-2864] - Normal biopsies of deparaffinized mouse skeletal muscle tissue.
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Applications for CACNA2D1 Antibody (20A)

Application
Recommended Usage

Flow Cytometry

1:100 - 1:200

Immunocytochemistry/ Immunofluorescence

1:250

Immunohistochemistry

1:500

Immunohistochemistry-Frozen

1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:500
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID: 8070639).

Reviewed Applications

Read 1 review rated 5 using NB120-2864 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CACNA2D1

FUNCTION: The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling.; Subcellular location: Membrane; Single-pass type I membrane protein; Tissue specificity: Central nervous system, skeletal muscle and aorta tissues.; Miscellaneous: Binds gabapentin, an antiepileptic drug.

Long Name

Voltage-dependent calcium channel subunit alpha-2/delta-1

Alternate Names

CACNL2A, CCHL2A, MHS3

Entrez Gene IDs

781 (Human); 12293 (Mouse); 25399 (Rat); 100009105 (Rabbit)

Gene Symbol

CACNA2D1

UniProt

P13806 (Rabbit)

Additional CACNA2D1 Products

Product Documents for CACNA2D1 Antibody (20A)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CACNA2D1 Antibody (20A)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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