CACYBP Antibody (2E3)
Novus Biologicals, part of Bio-Techne | Catalog # H00027101-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2E3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CACYBP (AAH05975, 1 a.a. ~ 228 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASEELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQKSQKKAELLDNEKPAAVVAPITTGYTVKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLNGKSYSMIVNNLLKPISVEGSSKKVKTDTVLILCRKKVENTRWDYLTQVEKECKEKEKPSYDTETDPSEGLMNVLKKIYEDGDDDMKRTINKAWVESREKQAKGDTEF
Reactivity Notes
Human. Other species not tested.
Specificity
CACYBP - calcyclin binding protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for CACYBP Antibody (2E3)
![Western Blot: CACYBP Antibody (2E3) [H00027101-M01]](https://resources.bio-techne.com/images/products/CACYBP-Antibody-2E3-Western-Blot-H00027101-M01-img0005.jpg "Western Blot: CACYBP Antibody (2E3) [H00027101-M01]")
Western Blot: CACYBP Antibody (2E3) [H00027101-M01]
Western Blot: CACYBP Antibody (2E3) [H00027101-M01] - Analysis of CACYBP expression in transfected 293T cell line by CACYBP monoclonal antibody (M01), clone 2E3.Lane 1: CACYBP transfected lysate(26.61 KDa).Lane 2: Non-transfected lysate.![Proximity Ligation Assay: CACYBP Antibody (2E3) [H00027101-M01]](https://resources.bio-techne.com/images/products/CACYBP-Antibody-2E3-Proximity-Ligation-Assay-H00027101-M01-img0003.jpg "Proximity Ligation Assay: CACYBP Antibody (2E3) [H00027101-M01]")
Proximity Ligation Assay: CACYBP Antibody (2E3) [H00027101-M01]
Proximity Ligation Assay: CACYBP Antibody (2E3) [H00027101-M01] - Analysis of protein-protein interactions between SKP1 and CACYBP. HeLa cells were stained with anti-SKP1 rabbit purified polyclonal 1:1200 and anti-CACYBP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: CACYBP Antibody (2E3) [H00027101-M01] - Detection limit for recombinant GST tagged CACYBP is approximately 1ng/ml as a capture antibody.
Applications for CACYBP Antibody (2E3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CACYBP
Alternate Names
CacyBP, calcyclin binding protein, GIG5, hCacyBP, MGC87971, RP1-102G20.6, S100A6-binding protein, S100A6BPgrowth-inhibiting gene 5 protein, Siah-interacting protein, Siah-interacting protein (SIP), SIPcalcyclin-binding protein
Entrez Gene IDs
27101 (Human)
Gene Symbol
CACYBP
OMIM
606186 (Human)
UniProt
Additional CACYBP Products
Product Documents for CACYBP Antibody (2E3)
Product Specific Notices for CACYBP Antibody (2E3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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