CACYBP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49922

Novus Biologicals, part of Bio-Techne
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NBP1-49922
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CACYBP Antibodies »

Product Summary for CACYBP Antibody

Immunogen

The immunogen for this product maps to a region between residue 200 and 228 of human Calcyclin Binding Protein using the numbering given in entry NP_055227.1 (GeneID 27101).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CACYBP Antibody

Western Blot: CACYBP Antibody [NBP1-49922]

Western Blot: CACYBP Antibody [NBP1-49922]

Western Blot: CACYBP Antibody [NBP1-49922] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CacyBP antibody NBP1-49922 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody NBP1-49921, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Immunohistochemistry: CACYBP Antibody [NBP1-49922]

Immunohistochemistry: CACYBP Antibody [NBP1-49922]

Immunohistochemistry: CACYBP Antibody [NBP1-49922] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- CacyBP used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for CACYBP Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2-5 mcg/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CACYBP

The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Alternate Names

CacyBP, calcyclin binding protein, GIG5, hCacyBP, MGC87971, RP1-102G20.6, S100A6-binding protein, S100A6BPgrowth-inhibiting gene 5 protein, Siah-interacting protein, Siah-interacting protein (SIP), SIPcalcyclin-binding protein

Gene Symbol

CACYBP

Additional CACYBP Products

Product Documents for CACYBP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CACYBP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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