CAD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61613

Novus Biologicals, part of Bio-Techne
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NB100-61613
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CAD Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1650 and 1700 of human carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase using the numbering given in entry NP_004332.2 (GeneID 790).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CAD Antibody

Western Blot: CAD Antibody [NB100-61613]

Western Blot: CAD Antibody [NB100-61613]

Western Blot: CAD Antibody [NB100-61613] - Detection of Human and Mouse CAD on HeLa whole cell lysate using NB100-61613 . CAD was also immunoprecipitated by rabbit anti-CAD antibodies NB100-61612 and NB100-61614.
Immunohistochemistry: CAD Antibody [NB100-61613]

Immunohistochemistry: CAD Antibody [NB100-61613]

Immunohistochemistry: CAD Antibody [NB100-61613] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CAD used at a dilution of 1:200 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .

Applications for CAD Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CAD

CAD is an enzyme involved in the first three steps of the de novo synthesis of pyrimidine nucleotides. CAD exhibits multiple catalytic activities and is described as a

Alternate Names

CAD protein, CAD trifunctional protein, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, anddihydroorotase, EC 2.1.3.2, EC 3.5.2.3, EC 6.3.5, EC 6.3.5.5, multifunctional protein CAD

Entrez Gene IDs

790 (Human); 69719 (Mouse)

Gene Symbol

CAD

UniProt

P27708 (Human)

Additional CAD Products

Product Documents for CAD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CAD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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