CAD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-61614

Novus Biologicals, part of Bio-Techne
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NB100-61614
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all CAD Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1850 and 2000 of human carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase using the numbering given in entry NP_004332.2 (GeneID 790).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CAD Antibody

Western Blot: CAD Antibody [NB100-61614]

Western Blot: CAD Antibody [NB100-61614]

Western Blot: CAD Antibody [NB100-61614] - Whole cell lysate (50 ug) from HEK293T, K-562, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAD antibody NB100-61614 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: CAD Antibody [NB100-61614]

Immunohistochemistry-Paraffin: CAD Antibody [NB100-61614]

Immunohistochemistry-Paraffin: CAD Antibody [NB100-61614] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CAD used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: CAD Antibody [NB100-61614]

Immunoprecipitation: CAD Antibody [NB100-61614]

Immunoprecipitation: CAD Antibody [NB100-61614] - Detection of human CAD by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAD antibody NB100-61614 (lot NB100-61614-3) used for IP at 6 ug per reaction. CAD was also immunoprecipitated by a previous lot of this antibody (lot NB100-61614-2) and another rabbit anti-CAD antibody. For blotting immunoprecipitated CAD, NB100-61614 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for CAD Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CAD

Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis (1). This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway (2). Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus (3). While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD (4-5).

Alternate Names

CAD protein, CAD trifunctional protein, carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, anddihydroorotase, EC 2.1.3.2, EC 3.5.2.3, EC 6.3.5, EC 6.3.5.5, multifunctional protein CAD

Entrez Gene IDs

790 (Human); 69719 (Mouse)

Gene Symbol

CAD

UniProt

NP_004332.2 (Human)

Additional CAD Products

Product Documents for CAD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CAD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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