Cadherin-11 Antibody (1A5)

Novus Biologicals, part of Bio-Techne | Catalog # H00001009-M04

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Flow Cytometry, Functional, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1A5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

CDH11 (P55287, 54 a.a. ~ 612 a.a) partial recombinant protein with Fc tag at C terminal. MW of the Fc tag alone is 50 KDa. 0

Specificity

This product is specific for Human CDH11 monoclonal antibody (M04), clone 1A5 [Gene ID: 1009].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cadherin-11 Antibody (1A5)

Western Blot: Cadherin-11 Antibody (1A5) [H00001009-M04]

Western Blot: Cadherin-11 Antibody (1A5) [H00001009-M04] - Analysis of CDH11 expression in human ovarian cancer.

Flow Cytometry: Cadherin-11 Antibody (1A5) [H00001009-M04]

Flow Cytometry: Cadherin-11 Antibody (1A5) [H00001009-M04] - Analysis of negative control PC3-MM2 cells only, mock control cells staining with 2nd antibodies and CDH11 stained PC3-MM2 cells.

Western Blot: Cadherin-11 Antibody (1A5) [H00001009-M04]

Western Blot: Cadherin-11 Antibody (1A5) [H00001009-M04] - Analysis of full length CDH11 over expression in L cell using CDH11 monoclonal antibody (M03), clone 1A5, and immunoblotted with CDH11 monoclonal antibody (Zymed).

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Applications for Cadherin-11 Antibody (1A5)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Functional study: Effective in blocking cadherin-11-mediated cell aggregation.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cadherin-11

Cadherin-11 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. Cadherin-11 is expressed in osteoblasts and the brain as well as other tissues.Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues.

Alternate Names

Cadherin11, CDH11, CDHOB, OB-Cadherin

Gene Symbol

CDH11

UniProt

P55287 (Human)

Additional Cadherin-11 Products

Product Documents for Cadherin-11 Antibody (1A5)

Product Datasheets

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COA

SDS

Product Specific Notices for Cadherin-11 Antibody (1A5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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