Calbindin D-28K Antibody (HL2378) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25391
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2378
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Calbindin D-28K Antibody (HL2378) - Azide and BSA Free
Immunogen
Recombinant fragment of human Calbindin D-28K.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Calbindin D-28K Antibody (HL2378) - Azide and BSA Free
Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] -
Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] -
Western Blot: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Human tissue extract (5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] -
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody (HL2378) - Azide and BSA Free [NBP3-25391] - Calbindin antibody [HL2378] detects Calbindin protein by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Calbindin stained by Calbindin antibody [HL2378] (NBP3-25391) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for Calbindin D-28K Antibody (HL2378) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Frozen
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Calbindin D-28K
Alternate Names
CAB27, CALB, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, Calbindin D28, D-28K, RTVL-H protein, Vitamin D-dependent calcium-binding protein, avian-type
Gene Symbol
CALB1
Additional Calbindin D-28K Products
Product Documents for Calbindin D-28K Antibody (HL2378) - Azide and BSA Free
Product Specific Notices for Calbindin D-28K Antibody (HL2378) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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