Calbindin D-28K Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99063

Novus Biologicals, part of Bio-Techne
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NBP2-99063-50ul
NBP2-99063-100ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Summary for Calbindin D-28K Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Calbindin D-28K.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Calbindin D-28K Antibody

Western Blot: Calbindin D-28K Antibody [NBP2-99063]

Western Blot: Calbindin D-28K Antibody [NBP2-99063]

Western Blot: Calbindin D-28K Antibody [NBP2-99063] - Anti-Calbindin D-28K rabbit polyclonal antibody at 1:500 dilution. Lane A: Mouse kidney tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 27 kDa
Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063]

Immunocytochemistry/ Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063]

Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-99063] - Immunofluorescence staining of Calbindin D-28K in 293 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human Calbindin D-28K polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063]

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063]

Immunohistochemistry-Paraffin: Calbindin D-28K Antibody [NBP2-99063] - Immunochemical staining of human Calbindin D-28K in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
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Applications for Calbindin D-28K Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calbindin D-28K

Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. Calbindin antibody is often used as a marker for cerebellum Purkinje cells.

Alternate Names

CAB27, CALB, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, Calbindin D28, D-28K, RTVL-H protein, Vitamin D-dependent calcium-binding protein, avian-type

Gene Symbol

CALB1

Additional Calbindin D-28K Products

Product Documents for Calbindin D-28K Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Calbindin D-28K Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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