Calcineurin A Antibody (5I6H8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16381

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16381-20ul
NBP3-16381-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5I6H8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Calcineurin A Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 422-521 of human Calcineurin A (Q08209). TLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calcineurin A Antibody (5I6H8)

Western Blot: Calcineurin A Antibody (5I6H8) [NBP3-16381]

Western Blot: Calcineurin A Antibody (5I6H8) [NBP3-16381]

Western Blot: Calcineurin A Antibody (5I6H8) [NBP3-16381] - Western blot analysis of extracts of various cell lines, using Calcineurin A Rabbit mAb (NBP3-16381) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for Calcineurin A Antibody (5I6H8)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calcineurin A

Calcineurin, a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. This metalloenzyme, also known as phosphatase 2B, is a heterodimer composed of a calmodulin binding, catalytic alpha subunit 61 kDa, calcineurin A) and calcium binding beta subunit (18 kDa, calcineurin B). The Ca2+ binding subunit, calcineurin B, is immunologically conserved from yeast to mammals. The presence of 2 different calcineurin B isoforms (beta 1 and beta 2) has been reported in rat testis. The catalytic subunit of calcineurin, calcineurin A, isolated from different tissues or different organisms, exhibits some immunological heterogeneity. Further, there are at least 2 isoforms of calcineurin A in bovine brain (alpha 1 and alpha 2, 61 and 59 kDa, respectively).

Alternate Names

CALNA, CNA1, PP2B, PP3CA, PPP2B, PPP3CA

Gene Symbol

PPP3CA

Additional Calcineurin A Products

Product Documents for Calcineurin A Antibody (5I6H8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calcineurin A Antibody (5I6H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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