Calcitonin Antibody (CALCA/3310)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13800

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # CALCA/3310

Concentration

0.2 mg/ml

View all Calcitonin Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Calcitonin protein (around aa 3-116) (exact sequence is proprietary) (Uniprot: P01258 )

Localization

Cytoplasm, Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

14.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14041)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Calcitonin Antibody (CALCA/3310)

Immunohistochemistry-Paraffin: Calcitonin Antibody (CALCA/3310) [NBP3-13800]

Immunohistochemistry-Paraffin: Calcitonin Antibody (CALCA/3310) [NBP3-13800]

Immunohistochemistry-Paraffin: Calcitonin Antibody (CALCA/3310) [NBP3-13800] - Formalin-fixed, paraffin-embedded human thyroid stained with Calcitonin antibody (CALCA/3310). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Calcitonin Antibody (CALCA/3310) [NBP3-13800]

Immunohistochemistry-Paraffin: Calcitonin Antibody (CALCA/3310) [NBP3-13800]

Immunohistochemistry-Paraffin: Calcitonin Antibody (CALCA/3310) [NBP3-13800] - Formalin-fixed, paraffin-embedded human thyroid stained with Calcitonin antibody (CALCA/3310). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Immunohistochemistry-Paraffin: Calcitonin Antibody (CALCA/3310) [NBP3-13800]

Immunohistochemistry-Paraffin: Calcitonin Antibody (CALCA/3310) [NBP3-13800]

Immunohistochemistry-Paraffin: Calcitonin Antibody (CALCA/3310) [NBP3-13800] - Formalin-fixed, paraffin-embedded human thyroid stained with Calcitonin antibody (CALCA/3310). Inset: PBS instead of primary antibody; secondary only negative control.
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Applications for Calcitonin Antibody (CALCA/3310)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:20
Application Notes
Immunohistochemistry (Formalin-fixed): 1:10-1:20 for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Calcitonin

Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

Long Name

Calcitonin Related Polypeptide Alpha

Alternate Names

CALC1, CALCA, Katacalcin, PCT

Gene Symbol

CALCA

UniProt

P01258

Additional Calcitonin Products

Product Documents for Calcitonin Antibody (CALCA/3310)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calcitonin Antibody (CALCA/3310)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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